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We had isolated a nonfermentative bacterium which shows a lemon-yellow nondiffusible pigment.After 48hrs of incubation at 30oC, a greenish pigment could also be seen.we suspect it to be Agrobacterium yellow group.Since we rely completely on manual methods of identification, what are the possible features for identifying this organism, or does any other nonfermenter produce this peculiar pigment(a mix of lemon-yellow and green)?
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There are many NFB that can produce the pigmented colonies that you describe. The most common are Stenotrophomonas maltophilia and members of the B. cepacia complex. If you have ruled out these two groups and the organism is motile then you have to consider P. luteola, P. orizhabitans (both oxidase negative), Sphingomonas paucimobilis, Agrobacterium Yellow Group, Balneatrix alpica, Massilia timonae and CDC Groups O-1 and O-2. If organism is non-motile then you have to consider Elizabethkingia meningoseptica, Chryseobacterium species, Myroides sp. and Sphingobacterium species. The best resource for up-to-date identification tables is Koneman's Color Atlas and Textbook of Diagnostic Microbiology, 6th ed. 2006, Chap. 7, Lippincott Williams & Wilkins. There is an electronic identification guide available at pschreck.com.
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