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What is your opinion on the use of a broth medium (such as thioglycollate) to replace "regular" anaerobic culture on selected sterile body fluids, such as pleural, synovial, CSF? If this seems to be an acceptable practice, should we be holding the broth for 3 days or 5? Also, what about sterile fluids that are more likely to exhibit growth, such as abdominal?
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Normally sterile body fluids which do not appear purulent can be inoculated in just a broth medium or even a blood culture bottle. Incubation should be for a minimum of 5 days in case unusual, slower growing anaerobes are present. CSF from patients with shunts may grow Propionibacterium spp which can colonize foreign material and cause chronic infection.
Abdominal fluid (excluding ascitic fluid) is usually collected from patients with peritonitis and will likely yield several types of organisms. This should be plated on standard anaerobic media including LKV and BBE to help identify members of the Bacteroides fragilis group.
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