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	When performing D Tests on purity plates, is the inoculum too diluted to give an accurate value? The final concentration tested isn't a .5 Mc Farland if we test from a prompt using Micrscan panels. Thanks
	There was a paper presented at the 2005 ASM meeting by Janet Hindler (abstract C-325) that compared the performance of a Microscan Prompt inoculation method to the CLSI recommended method for D testing on 100 S. aureus isolates (80 MRSA and 20 MSSA). The results showed comparable results. The prompt method involved using a 0.01 ml loop to collect a sample from the inoculum tray after the MIC tray was inoculated. The loop was used to streak one half of a BAP confluently. The remaining portion of the plate was streaked for isolation (purity check). Ery and Clind disks were applied 13mm edge to edge to the confluent growth streak area and plates incubated. She reported 100% agreement with the CLSI method. Try it! DISCLAIMER Askit is for general information purposes only and may not be relied upon by users for decisions or action in specific circumstances. Neither ASM nor the expert assures the accuracy or completeness of information provided. Questions are answered based on current information, which may change over time. Please note the date that a question was answered. If an answer is more than one year old, you may want to resubmit the question for an updated answer. The advice and comments do not necessarily reflect the views of ASM. ASM and INDIVIDUAL experts expressly disclaim any and all liability for any direct, indirect, or special damages or loss of any nature incurred as a consequence of use of this site or any advice or comment by any expert. By using Askit, the user accepts the waiver of claims and all terms of use of ASM�s site, including the disclaimers set forth in the �Legal Rights� link on ASM�s home page.

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