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Related to my question from yesterday - if BEA agar with 8 mcg/ml vanco is used to screen for VRE, should EACH black colony that grows be subbed for MICs? Seems like a lot of work, but I don't think one could assume that they are all the same. Would it be reasonable to screen the subs for motility and pigment and do MICs on all non-motile, non-pigmented?
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In order to differentiate enterococci with vanA or vanB resistance genes (E. faecalis & E. faecium) from those with vanC which is expressed vancomycin MIC's in the range 0f 6-8 mcg/mL you would have to identify the organisms growing on this screening medium. Use of a screening medium with 10mcg/mL of vancomycin would eliminate this step (Manual of Clinical Microbiology, 8th edition, Chapter 74).
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