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First of all, you did not say what type of specimens are tested in your laboratory. For urine specimens you could get away without using CO2. For all other specimens you need a CO2 environment to grow many of the possible pathogens. Also if your testing volume is small, you do not have to have a CO2 incubator. You can use an anaerobic jar with a CO2 generator envelope. Also you can use a older method, that has less control, which is a candle jar. You put your plates in it. Light a candle and seal the jar. The candle burns off the oxygen and creates CO2. Finally, if you are doing susceptibility testing, your incubator should be set at 35 C. No higher.
Micheal Gaydos
Cleveland VAMC Cleveland
vhaclegaydoj@med.va.gov
216 791 3800 x4920
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