ASM Awards and Grants


	HI! MY QUESTION IS : WE ARE CURRENTLY USING MICROSCAN PROMPT SYSTEM (BREAKPOINT COMBO PANELS). MY QUESTION HAS SEVERAL PARTS.THE FIRST QUESTION IS ON THE 30-ug CEFOXITIN DISC AND TESTING FOR MEC �A MEDIATED RESISTANCE IN STAPHYLOCOCCI.. THE WAY I AM READING CLSI STANDARDS IS WE NEED TO TEST ONLY COAGULASE NEGATIVE STAPHYLOCOCCUS (NOT STAPH EPIDERMIDIS) WITH CEFOTOXIN DISC FOR MEC A MEDIATED RESISTANCE. THAT STAPHYLOCOCCUS EPIDERMIDIS AND STAPHYLOCOCCUS AUREUS NEED NOT BE TESTED BECAUSE MICROSCAN MIC FOR OXACILLIN CORRELATES WITH THE PRESENCE OR ABSENCE OF THE GENE ENCODING METHICILLIN RESISTANCE (MecA). IS THIS CORRECT? MY SECOND QUESTION IS WHEN I START USING THE CEFOXITIN DISC I WILL NEED TO VALIDATE IT. DOES THIS MEAN TESTING IT FOR 20-30 DAYS USING STAPHYLOCOCCUS AUREUS ATCC 29213 OR DOES IT MEAN HAVING MY RESULTS DUPLICATED BY ANOTHER LABORATORY. IF IT IS THE LATTER OF THESE TWO HOW MANY RESULTS DO YOU HAVE TO HAVE DUPLICATED. I HAVE A FEELING I NEED TO DO BOTH OF THESE PROCEDURES. IF I HAVE TO DO THE 20-30 QC TESTING WOULD YOU PLEASE TELL ME IF THE NUMBER OF DAYS HAS CHANGED FROM 30 TO 20 AND WHEN THIS HAPPENED AND WHERE IT IS WRITTEN IN CLSI STANDARDS.. I THINK NEW YORK STATE REQUIRES US TO DO 30 DAYS. WHEN I PERFORM THE CEFOXITIN DISC PROCEDURE ON STAPHYLO- COCCUS DO I PERFORM IT USING THE STANDARD KIRBY BAUER METHODOLOGY AND THEN INTREPRET IT ACCORDING TO CLSI (M100-S16) PG 123 LAST QUESTION! IF I DO ALL MY STAPHYLOCOCCUS WITH CEFOXITIN THEN I WOULD BE ABLE TO ELIMINATE THE OX-6 PLATE. IS THIS WHAT MOST LABORATORIES ARE DOING NOW ? OR ARE THEY DOING JUST THE COAGULASE NEGATIVE STAPHYLOCOCCUS (NOT STAPHYLOCOCCUS EPIDERMIDIS) AND NOT STAPH AUREUS, WHEREBY YOU WOULD WANT TO KEEP THE OX-6 PLATE. AT OUR LABORATORY WE ONLY USE THE OX-6 PLATE FOR STAPH AUREUS TO CONFIRM MRSA. I KNOW OF SOME LABORATORIES THAT USE THE OX-6 PLATE FOR ALL STAPHYLOCOCCUS. IS THIS CORRECT? THANK YOU FOR YOUR HELP.
	HI! MY QUESTION IS : WE ARE CURRENTLY USING MICROSCAN PROMPT SYSTEM (BREAKPOINT COMBO PANELS). MY QUESTION HAS SEVERAL PARTS.THE FIRST QUESTION IS ON THE 30-ug CEFOXITIN DISC AND TESTING FOR MEC �A MEDIATED RESISTANCE IN STAPHYLOCOCCI.. THE WAY I AM READING CLSI STANDARDS IS WE NEED TO TEST ONLY COAGULASE NEGATIVE STAPHYLOCOCCUS (NOT STAPH EPIDERMIDIS) WITH CEFOTOXIN DISC FOR MEC A MEDIATED RESISTANCE. THAT STAPHYLOCOCCUS EPIDERMIDIS AND STAPHYLOCOCCUS AUREUS NEED NOT BE TESTED BECAUSE MICROSCAN MIC FOR OXACILLIN CORRELATES WITH THE PRESENCE OR ABSENCE OF THE GENE ENCODING METHICILLIN RESISTANCE (MecA). IS THIS CORRECT? HI, The cefoxitin disk test provides a better test result that oxacillin disk tests and can help laboratories that choose to back up automated test methods with another test. You are correct in assuming that you are most likely to detect mecA negative coagulase negative staphylococci that test resistant by current CLSI breakpoints for coagulase negative staphylococci. MY SECOND QUESTION IS WHEN I START USING THE CEFOXITIN DISC I WILL NEED TO VALIDATE IT. DOES THIS MEAN TESTING IT FOR 20-30 DAYS USING STAPHYLOCOCCUS AUREUS ATCC 29213 OR DOES IT MEAN HAVING MY RESULTS DUPLICATED BY ANOTHER LABORATORY. IF IT IS THE LATTER OF THESE TWO HOW MANY RESULTS DO YOU HAVE TO HAVE DUPLICATED. I HAVE A FEELING I NEED TO DO BOTH OF THESE PROCEDURES. IF I HAVE TO DO THE 20-30 QC TESTING WOULD YOU PLEASE TELL ME IF THE NUMBER OF DAYS HAS CHANGED FROM 30 TO 20 AND WHEN THIS HAPPENED AND WHERE IT IS WRITTEN IN CLSI STANDARDS.. I THINK NEW YORK STATE REQUIRES US TO DO 30 DAYS. WHEN I PERFORM THE CEFOXITIN DISC PROCEDURE ON STAPHYLO- COCCUS DO I PERFORM IT USING THE STANDARD KIRBY BAUER METHODOLOGY AND THEN INTREPRET IT ACCORDING TO CLSI (M100-S16) PG 123 LAST QUESTION! IF I DO ALL MY STAPHYLOCOCCUS WITH CEFOXITIN THEN I WOULD BE ABLE TO ELIMINATE THE OX-6 PLATE. IS THIS WHAT MOST LABORATORIES ARE DOING NOW ? OR ARE THEY DOING JUST THE COAGULASE NEGATIVE STAPHYLOCOCCUS (NOT STAPHYLOCOCCUS EPIDERMIDIS) AND NOT STAPH AUREUS, WHEREBY YOU WOULD WANT TO KEEP THE OX-6 PLATE. AT OUR LABORATORY WE ONLY USE THE OX-6 PLATE FOR STAPH AUREUS TO CONFIRM MRSA. I KNOW OF SOME LABORATORIES THAT USE THE OX-6 PLATE FOR ALL STAPHYLOCOCCUS. IS THIS CORRECT? THANK YOU FOR YOUR HELP. Hi,

Return to Questions