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Since the current CLSI(NCCLS) M11-A6 document states that the Micro Broth Dilution technique can only be used to test susceptibility for B fragilis Group organisms, what does a small community hospital do when other anaerobes grow from sterile sites and a sens needs to be done? Our reference lab only offers a broth dilution MIC, and to send an organism out means a 2-4 day wait for results.Will the new CLSI Anaerobe susceptibility document allow for more organisms to be tested with the broth dilution method?
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The broth dilution method set up on the bench introduces a lot of oxygen into the test, and this kills many of the fastidious anaerobes faster that the antibiotic being tested, producing unreliable results.
For small labs doing limited anaerobe work, the Etest is the simplest and most reliable method for testing anaerobes. CLSI does not discuss proprietary systems (Vitek, Microscan, Etest, etc) in their documents, but "gradient methods" mean the Etest, and this is suggested as an alternative method in the document.
The Etest is very rapid and many anaerobes will grow after overnight incubation. If you don't have a large anaerobe jar for the 150 mm plates, you can use the standard 100 mm plates and place 2 strips with the high concentration ends opposing each other.
Good luck!
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