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E. faecalis ATCC 29212 or E. faecalis ATCC 33186 may be used to ensure Mueller Hinton (MH) agar does not contain an excessive amount of thymidine. This step is primarily for manufacturers of MH agar, including laboratories that make their own MH agar.
For laboratories which purchase MH agar, zone diameters of sulfonamides and trimethoprim should be carefully monitored. If problems occur with quality control of the disks, it may be necessary to test with one of the E. faecalis QC strains. Satisfactory media will provide essentially clear, distinct zones of inhibition >=20 mm (with either QC strain). Unsatisfactory media will produce no zone of inhibition, growth within the zone, or a zone of <20 mm. (See CLSI document M2-A9 7.1.4, page 8).
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